ARI Orthopaedics 2026, Collection 1

Cartilage and Disc

Meeting Abstracts:

Oral presentations: click here to download all the abstracts in one file

Poster presentations: click here to download all the abstracts in one file

Oral presentations

1. Xudong Joshua Li
A novel platform for investigating neuroimmune interactions in degenerative disc disease

2. Martin Englund
Osteoarthritis – an evolutionary mismatch disease?

3. D Sheyn, R Lev-Gur,N More, N Mahmoudi,J Sheyn,D Roell,W Tawackoli
Temporal proteomic profiling of PTOA identifies the optimal therapeutic window for anti inflammatory cell therapy

4. Catherine Le Visage
Biofabrication of the intervertebral disc: progress and remaining challenges

5. Julian Jones
3D printed “Bouncy Bioglass” can regenerate articular cartilage focal defects

6. A Damle, D Mock, B Dasen, K Sovdagarova, L Sauteur, SR Roig Merino, T Welting, A Garcia-Garcia, P Ismailidis, F Imhoff, I Martin, A Mainardi, A Barbero
A nasal chondrocyte-based inflammation resistant therapy for osteoarthritis

7. A Kumar, P Torabi Rahvar, A Bouduban, M Wöltje, B Gantenbein
Biomimetic composite hydrogel using silk fibres, hyaluronic Acid, and Collagen type II for nucleus pulposus regeneration

8. Chris Little
Joint injury: New insights from animal models on injury risk and long-term consequences

9. Giuseppe Filardo
Cartilage regeneration and joint preservation: A challenging translational journey to the clinical practice

10. TP Schaer, OM Swiber, RM Butler, A Winslow, M Seraydarian, C Durst, CM Snyder, AF Chen, NJ Hickok
The immediate postoperative joint milieu is predominantly alkaline under normal and staphylococcal septic conditions

11. G Rösch, M Pfeiffer, PL Tsai, M Gold, F Zaucke, D Slattery, Z Jenei-Lanz
Chronic stress-induced spatiotemporal alterations in corticosterone converting enzymes and adrenergic receptors in experimental osteoarthritis

12. Rui Yue
Identification of novel skeletal stem/progenitor cells in the perichondrium

13. Makarand Risbud
Central role of pyrophosphate metabolism in spine health

14. A Schwab, C Schneider, S Gylstorf, C Göttsch, AHalm-Pozniak, CH Lohmann, J Bertrand
Extracellular vesicles from spontaneously calcifying chondrocytes stimulate chondrocyte and matrix calcification

15. RM Gonçalves, S Pilão, AM Martins,J Caldeira, L Teixeira
Unlocking CD44+ intervertebral disc cells in homeostasis and degeneration for therapeutic targets in discogenic pain

16. H Lyu, C Li, F Yuan, T Zhang, J Zhou
Mechanically preconditioned synovial mesenchymal stem cells organoid exosomes for osteoarthritis repair

17. M Muerner, RV Balasubramanian, J Fernández-Pérez, A Ovsianikov, S Grad
Mesenchymal stromal cell spheroid-loaded microscaffolds for intervertebral disc repair

18. Martin Knight
Organ-on-a-chip models of inflammatory joint disease

19. Jérôme Noailly
From people to molecules: In Silico insights for precision health in spine and synovial joints

20. H Meng, MJ Stoddart, S Grad, Z Li
Pericellular matrix restoration enhances resistance of chondrocytes to inflammatory and mechanical stress

21. K Wuertz-Kozak, J Hasler
Exploring TRPV4 mechanosensitivity to substrate stiffness and mechanical loading in annulus fibrosus

22. I Zulkiflee, Z Razak, H Revill, A Pandit, ILM Isa
Human disc-on-a-chip: modelling human intervertebral disc degeneration via microfluidic and hydrogel-based system

23. R Narcisi, A Bertocci, S Costa, A Flier, L You, P Chiafele, M Ghazvini, H Ray-Jones G van Osch, MP Chien, J de Pinho Gonçalves, J van Meurs
The “village-in-a-dish” approach as an innovative cellular model for investigating variability in response to loading in chondrogenic cells

24. Rachel E Miller
Imaging neuroplasticity in knee osteoarthritis

25. Jiao Jiao Li
The potential of MSCs and MSC-EVs as new therapeutics for osteoarthritis

26. J Ma, J Müller, T Serra, S Grad
A neurovascular disc system to study the role of endothelial cells in nociceptive axonal growth

27. S Dudli, J Devan, J Zhou, P Wu, N Bonnheim, C O’Neill, JC Lotz, TM Link, AJ Fields
Intradiscal propionic acid levels in chronic low back pain patients (CLBP) with Modic type 1 changes (MC1) are associated with systemic immune cell activation

28. Marianna Tryfonidou
Notochordal cells lead the way to regeneration

29. Nam Vo
Filth or fuel? Lactate’s role in disc metabolism and epigenetic regulation

30. SM Richardson, CT Dube, EM Oldfield, SM Baker, M Buckley, R Jennings, JA Hoyland
High-resolution spatial transcriptomic and proteomic mapping of the developing human intervertebral disc

31. CL Dahia, A Upadhyay, G Belen
Direct In Vivo capture of the nucleus pulposus secretome

32. Zhen Li
Advancing osteoarthritis therapy: In Vitro and Ex Vivo modeling in bioreactor platforms

33. Tim Welting
Exploring the bioactivity of synovial fluid in osteoarthritis as a vector for intra-joint biomolecular cross-talk

34. MC Cordeiro, AG Guex, JK Wychowaniec, A Barbero, MJ Stoddart
Dynamic compression strain drives chondrogenic transcriptional fate through actin cytoskeleton dynamics, TGF-ß/SMAD signalling and YAP1 translocation

35. FK Ciliberti, I Maruotto, HR Jónsson, P Gargiulo
Objective imaging biomarkers for knee OA: a data-driven view of cartilage degeneration

36. Ambika G Bajpayee
Charge-driven gene delivery to cartilage

37. SA Kamali, C Voskamp, LT Laagland, A Eversdijk, M Teunissen, S Plomp, A Tellegen, G Sponder, BP Meij, K Guse, S Plutizki, MA Tryfonidou
Protective effects of IL-1Ra gene therapy on intervertebral disc degeneration: In Vitro and In Vivo findings in beagle dogs

38. CL Le Maitre, G McDermott, D Swift, M Kim, D Jackson, JM Wilkinson
Ex Vivo exploration of PCRX-201 in human OA joint tissues

Poster Presentations

1. Longitudinal in vitro assessment of mechanical and compositional changes in cartilage under mechanical traumao inflammation and cyclic loading. RL Abeysekaral, A Saastamoinenr, K Honkää , J Lourdes Rios, P Tanska, MAJ Finnilä

2. Identification of repurposing drugs that protect chondrocytes from bcp crystals in osteoarthritis. JEJ Beaumont, MMJ Caron, A Cremers, M de Vries, GGH van den Akker, TJM Welting

3. Decellularized annulus fibrosus scaffolds for in vitro disease. AL Castro, M Macedo, JB Costa, RM Gongalves

4. Mechanical environment drives acetylation-mediated epigenetic remodeling in C28/I2 in chonodrocytes. R Castro-Vifluelas, DM Minton, I Jonkers

5. Biomimetic and injectable aminated collagen-hyaluronic acid-glycosaminoglycan (aCol-HA-GAG) biomaterial scaffold for cell-based intervertebral disc regeneration. M Chaaban, T Goel, X Yang, D Cher, J Guicheux, M Fusellier, BP Chan, C Le Visage

6. Using Mesendoderm Progenitor Cells Seeded in Hydrogel Biomaterial as a strategy to Regenerate the Intervertebral disc. C Cicione, RJ Williams, J Snuggs, J Warin, C Sammon, B Gatenbein, D Noel, JM LeMaitre, A Camus, MA Tryfonidou, G Vadala, C Le Maitre

7. Why does animal health matter - Correlation of postoperative welfare with clinical parameters in a rabbit model of knee osteoarthritis. RZ Coeti, M Carretero-Hernandez, Y Zhang, F Lir, JK Venkatesan, A Acheli, G Schmitt, M Cucchiarinir, H Madry

8. SHH'and' TBXT-mRNA-loaded lipid nanoparticles as a promising regenerative therapy for degenerated intervertebral disc. D Corraini, A Varela-Moreira, M Buiter, A Eversdijk, C Voskamp, Q Yang, Z Leiz, RM Schiffelers, MA Tryfonidou

9. Degenerate intervertebral disc environment impairs the regenerative potential of notochordal cell-derived extracellular vesicles. D Corraini, G Voskamp, A Eversdijk, FM Riemers, HR Vos, P Vader, GGH van den Akker, TJM Welting, K Ito, MHM Wauben, MA Tryfonidou

10. Extracellular Matrix Depletion Underlies Mechanical Dysfunction in the Aging Intervertebral Disc of Mouse. CL Dahia, AP Hallmark, S Bogdan, AM Ali

11. In Search of a Disease-modifying Osteoarthritis Drug Using Aptamer Technology LJM Deneer, MR Schlüter, K Warmink, HH Weinans, JL Rios

12. HLA-A*26:01 is associated with vertebral endplate bone marrow lesions (Modic changes): evidence for an immunogenetic component in spinal degeneration. J Devan, CJ Smith, J Määttää, J Zhous, P Leary, PH Wu, T Link, O Distler, J Karppinen, FMK Williams, A Fields, S Dudli

13. Modeling Neutrophil Responses to Annulus Fibrosus Secretomes Under Physiological and Inflammatory Conditions J Dong, E Bektas, E Bovay, L Buonarrivo, M Mürner, W Chen, J Ma, E Della Bella, Z Li, S Grad, M D'Ester

14. Molecular Patterns in Early Osteoarthritis: The Role of Cellular Senescence E Duman, G Pattappa, P Pann, S Reppenhagen, M Rudert, P Angele, D Docheva

15. Enhancing Chondrocyte Regulatory Network Accuracy for Multiscale Coupling via Integrated Signalling Pathways A Pascuet-Fontanet & J Noailly

16. A Dynamic Whole Knee Joint Culture that Preserves Cartilage Viability. S Funk, M Tryfonidou, L Utomo

17. Acetylcysteine regulates the endoplasmic reticulum stress response in CEP-like chondrocytes exposed to proinflammatory cytokines. L Gonzalez-Blanco, Y Farrag, M Farrag, J Pino, B Gantenbein, O Gualillo

18. Minimally Invasive Biologic Fusion: Synergistic EP4 and BMP-2/L51P Signaling Promotes Osteogenic Differentiation of Human Annulus Fibrosus Cells (Discfuse): Dose Optimization of KMN-159 L Gonzalez-Blanco, S Zwingenberger, X Tian ,C E Albers, B Gantenbein, S Häckel

19. Promoting Intervertebral Disc Fusion Through Combined BMP2, L51P, and EP4 Agonism: In Vitro Evidence from Human Annulus Fibrosus Cells (Discfuse). S Häckel, S Chen, L Gonzalez-Blanco, S Zwingenberger, X Tian, CE Albers, Spine Science Commission of the German Spine Society (DWG), B Gantenbein.

20. Sirtuin-6 Therapy: A Promising Concept with Limited Efficacy in Intervertebral Disc Degeneration in Beagle Dogs SA Kamali, C Voskamp, A Eversdijk, LT Laagland, M Teunissen, S Plomp, A Tellegen, GSponder, BP Meij, K Guse, S Plutizki, MA Tryfonidou

21. Cartilage-on-Chip reveals a translational response to mechanical cues. JJ Kreutzkamp, I van Galen, E Lauwers, D Surtel, M de Vries, A Cremers, C Marjolein, C Paggi, T Welting, G van den Akker

22. Biomimetic Composite Hydrogel using Silk Fibres, Hyaluronic Acid, and Collagen type ll for Nucleus Pulposus Regeneration. A Kumar, Parisa T Rahvar, A Bouduban, M Wöltje, B Gantenbein

23. Advanced Ex Vivo Model Recapitulating the Complexity of Disc Degeneration for pre-clinical Testing of a Next-Generation Gene Therapy. LT Laaglanot, SA Kamali, C Voskamp, E Salzer, G Sponder, K Guse, S Plutizki, K Ito, MA Tryfonidou

24. Human Fibroblast-like Synoviocytes Modulate the Inflammatory Response of Human Articular Chondrocytes Across Multiple Patients S Liu, D Terrazas, S Verbruggen, T Hopkins, M Knight

25. Sialylation ls a Central Mediator of Osmolarity- and pH-Dependent Ferroptosis and ER Stress in Nucleus Pulposus Cells. J Lyu, A Scheper, K Joyce, K Das, A Pandit

26. Single-Cel Resolution Attas of Glycosylation Heterogeneity in lntervertebral Disc Degeneration. J Lyu, P Rodriguez-Atonso, H Revill, A Pandit

27. Influence of Fibre Reinforcement on Annulus Fibrosus Mechanics in Finite Element Models. M Machado, R Gill, E Pegg, Gheduzzi

28. The actin-binding and -bundling protein plastin-3 (PLS3) and cartilage mechanotransduction. D Maehlich, F Busse, NM Kronenberg, M Vovchenko, N Vaes, H van Oosterwyck, MC Gather, A Niehoff

29. Deploying iPSC-derived M2 Macrophages to Mitigate PTOA in 2D, 3D and in vivo Models. N Mahmoudi, L Zila, J Sheyn, A Prasad, J Wechsler, S Mohyeddinipour, D Roell, M Bastani, M Orr, A Spitzer, S Rajaee, M Chavez, N More, R Lev Gur, W Tawackoli, D Sheyn

30. Evaluation of Clinically Relevant Lipid Nanoparticles for mRNA delivery in Osteoarthritic Joint Models. JAJ Martens, A Varela Moreira, A Eversdijk, R Schiffelers, MA Tryfonidou, L Utomo

31. Clinically Meaningful Machine Learning: The Role of Feature Selection in Knee Osteoarthritis. I Maruotto, I Jónsson, P Gargiulo

32. Bioactive And Load-Bearing Calcium Phosphates For Developing Biomimetic Spine Fusion Implants. BF Massel, R Truckenmüller, P Habibovic, ZN Tahmasebi Birgani

33. A High-Throughput Cartilage-on-Chip Model Reveals Mechanosensitive Chondrogenic Responses in Human MSCs. L Mecchi, MMJ Caron, TJM Welting, MJ Stoddart

34. Antibiotic-loaded hyaluronic acid-tyramine hydrogel to prevent bacteria-mediated disc degeneration and Modic type 1 changes of herniated discs. D Menghini, E Kuhn, M Muerner, O Distler, M Farshad, S Grad, M D’Este, TF Moriarty, JG. Snedeker, S Dudli

35. HTRAl-driven disc matrix fragmentation propagates Modic-like pathology across the disc-endplate-marrow axis. T Menigs, J Devan, A Nüesch, P Hatt, I Heggli, B Roschitzki, A Laimbacherr, N Herger, M Farshad, D Brunner, O Distler, S Dudli

36. Human-relevant chondroprogenitor-based models of cartilage injury to study regulated cell death after surgical debridement D Mock, M De Luise, A Barbero

37. CRISPR-Engineered MSC-Derived Extracellular Vesicles: From TSG-6 Overexpression to Subpopulation Characterization. L Moralesl, I Martinez-Zalbideal, L Brown, V Puvanesarajah, K Wuertz-Kozak

38. The Protective Effect of Microgel Cell Delivery for Intervertebral Disc Regeneration Therapy. N More, T Lee, A Prasad, S Mohyeddinipour, J Sheyn, G Kaneda, M Chavez, D Roell, L Zila, D Khorsandi, M Grol, M Dokmeci, D Seliktar, W Tawackol, D Sheyn

39. The Role and Mechanism of Matrix Sulfation in Regulating Chondrocyte Cell Cycle Abnormalities in Osteoarthritis B Ni, C Zheng, L Yang

40. Bacteria-induced Epigenetic Modification in Intervertebral Disc Cells. A Nüesch, E Ezen, T Mengis, P Bitterli, O Distler, C Seiler, S Dudli

41. I Like to move it: Robotic microsystem approaches for musculoskeletal disease modelling. M Rothbauer

42. Modelling Osteoclast-Chondrocyte Interactions in a 3D Human Organoid System. F Safari, S Grad, MJ Stoddart, Z Li

43. Synergistic Effects of Mechanical Loading and Inflammation on Chondrocyte Gene Expression in a Cartilage-on-Chip Model Z. Sarmadian, J Henriques, V. Peitso, CA Paggi, E Lauwers, M Risteli, A Mobasheri

44. Intra-articular a-MSH analogs attenuate cartilage degradation and systemic inflammation in a murine osteoarthritis model N Schäfer, P Pann, M Rudolph, N Pere, H Clausen-Schaumann, M Böhm, S Grässell

45. Application of an Electric Current in the Developing Chicken Embryo inducs Spinal Deformity. TP Schaer, JV Jordan, G Orlando, A Ketscheks, G Gallo, J Anari, BD Snyder. PJ Cahill

46. Impact of Digestion Time and Tissue Size on the Isolation of TIE2+ Nucleus Pulposus Progenitor Cells L Schlagenhof, A Stirnimann, F llle, B Gantenbein

47. A Comparison of Bovine Adipose-Derived Mesenchymal Stromal Cells With Nucleus Pulposus Progenitor Cells: Exploring Extracellular Vesicle Therapy for intervertebral disc Regeneration L Schlagenhof, C Raffaele, C Zivko, P Luciani, A Hashemi Gheinani, B Gantenbein

48. Mechanical loading of 3D-printed thermoplastic polyurethane scaffolds promotes chondrogenic differentiation of human mesenchymal stromal cells without exogenous TGF-ß1 while limiting hypertrophy. M Schlittler, L Mecch, MJ Stoddart

49. Bridging biomechanical and biochemical profiles in whole-organ Intervertebral Disc Degeneration Models D Schnyder, M Muerner, N Fischlin, C Honz, M Santolini, P Schwarzenberg, S Grad

50. Extracellular vesicles from spontaneously calcifying chondrocytes stimulate chondrocyte and matrix calcification A Schwab, C Schneider, S Gylstorf, C Gottsch, A Halm-Pozniak, CH Lohmann, J Bertrand

51. Deoxycholic Acid Links Gut Microbiota to a Novel Pro-Inflammatory Adipocyte Phenotype in Modic Type I Changes F Schulze, J Devan, T Mengis, LP Hatt, M Farshad, O Distler, S Dudli

52. Notochordal & mesendoderm progenitor cells as potential cell sources for JW Snuggs, S Basatvat, J van Maanen, M Templin, M Tryfonidou

53. Targeting cellular senescence to combat osteoarthritis and intervertebral disc degeneration J Snuggs, D Swift, H Wilson, E Kiss-Toth, JM Wilkinson, C Le Maitre

54. TGF-ß-driven Interleukin-6 production by human fibroblast-like synoviocytes is OA patient-specific and is regulated by multiple active sites within IL-6 promoter. JSJ Steijns, GGH van den Akker, RHML Stassen, LCW Peeters, MMJ Caron, TJM Welting

55. Impact of TGF-beta and physioxic culture conditions on periosteal chondrogenesis M Stewart, M Schlittler', S Fliick, M Stoddart

56. Optimizing Cartilage Tissue Engineering Using Simulated Microgravity and Pulsed Electromagnetic Fields A Stirnimann, R Bischof , G Cerretti, L Beeler, M Egli, F Ille

57. Targeting VGLL4 maintains extracellular matrix homeostasis and mitigates osteoarthritis. J Suo, Duo Wang2, J Wang

58. Single-cell Analysis identifies Lining Fibroblasts and Macrophages as Key Contributors to Persistent Synovial Inflammation after Cartilage Injury in Experimental Canine Osteoarthritis M Teunissen, FM Riemers, BAC Housmans, L Winkel, SGM Plomp, SCM Mastbergen, BP Meij, TJM Welting, MA Tryfonidou

59. Biomimetic Nucleus Pulposus-on-Chip for Intervertebral Disc Degeneration Research PT Rahvar, L Gonzalez-Blancol, B Gantenbein

60. Sirna-Lipid Nanoparticles Enable Extensive Gene Silencing In Primary Human Macrophages And Osteoarthritic Patient. Derived Chondrocytes. D Pereira de Vasconcelos, M Couto, E Bonvin, B Ribeiro, B Osorio de Valdoleiros, A Nobrega, A Moncada-Pazos, G Guerin, F Poulhes, S Slaoui, O Zelphati, M Ribeiro da Silva, D Dupin, M Lamghari

61. The effect of osteoarthritis on the peripheral nerves of the dorsal root ganglions in dogs. P Virankabutra, S Plomp, MA Tryfonidoul, M Teunissen

62. Interplay Between Canonical Wnt Signaling And A5b1 Integrins Modulates Mechanoresponse In Human Articular Cartilage. N Viudes-Sarrion, R Castro-Vinuelas, N Vaes, E Blain, S Monteagudo, R Lories, I Jonkers

63. Native extracellular vesicles derived from notochordal cell matrix contribute to a healthy nucleas pulposus cell state. C Voskamp, N Rijneveld, D Corraini, MHM Wauben, MA Tryfonidou

64. Notochordal cell-derived extracellular vesicles: a functional protein corona affecting regenerative signaling pathways. C Voskamp, JC van Maanen, FM Riemers, HR Vos, A Eversdijk, M Kleinjan, JC van Herwijnen, FC Bach, TJM Welting, CL le Maitre, GGH van den Akker, MHM Wauben, MA Tryfonidou

65. Large-scale proteomic / metabolomic analyses reveal new pathophysiological insights between Sleep behaviours and Osteoarthritis risk J Wang, Q Tong, R Shao, H Yuan

66. Oxysterol undersulfation promotes osteoarthritis by fueling chondrocyte lipid accumulation H Wang, B Ni, L Yang, C Zheng

67. Immediate-early dynamics of lineage-commitment, mechanotransductive and circadian gene expression in human MSCs in response to a single mechanical loading event. KS Wendrich, L Mecchi, QJ Meng, MJ Stoddart

68. Differences between histochemical glycosaminoglycan staining methods for cartilage tissues. RV Wolff, N Kops, E Salzer, R Narcisi, MSB Rathnayake, KS Stok, GJVM van Osch

69. Inflammatory cytokines alter the response to physiological loading in osteochondral explants. RV Wolff, E Salzer, N Kops, R Narcisi, GJVM van Osch

70. Decellularized Extracellular Matrix Particle-Based Biomimetic Hydrogel Preserves Cartilage Matrix Components and Attenuates Inflammation in a Human Osteochondral Explant Osteoarthritis Model J Xu, M D’Este, M Alini, S Grad, J Geurts, Z Li

71. Functional Complementation of Neonatal and Adult Intervertebral Disc ECM: An A&NM-dECM Scaffold for Enhanced Intervertebral Disc Degeneration Repair, F Yuan, C Li, T Zhang, J Zhou, J Hu, H Li

72. Redox Responses to Mechanical Loading are Heterogeneous in Human Cartilage C Yu, CR Henak

73. Combining Experimental Data and Mathematical Modeling To Interpret Glucose Transport and Metabolic Gradients In A Joint Tissue Co-Culture Model. J Zwicker, F Safari, J Stojkovska, P Petrovic, S Grad, MJ Stoddart, Z Li, B Obradovic